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. 2008 May 9;283(19):13009–13020. doi: 10.1074/jbc.M801222200

FIGURE 2.

FIGURE 2.

AMPK is necessary but not sufficient for glucose deprivation-induced protein O-GlcNAcylation. A, lysates from Neuro-2a cells glucose-deprived for the indicated times (following 30-min pretreatment with DMSO or 20 μm compound C) were immunoblotted (IB) for O-GlcNAc, OGT, pAMPK, AMPK, and actin. B, mRNA prepared from Neuro-2a cells glucose-deprived for the indicated times (following 30-min pretreatment with DMSO, 20 μm compound C, or 5 μg/ml actinomycin D) were subjected to qRT-PCR using primers specific for OGT and 18 S RNA. C, lysates from Neuro-2a cells treated for the indicated times with 5 mm metformin or glucose deprivation were immunoblotted for O-GlcNAc, OGT, pAMPK, AMPK, and actin. D, mRNA prepared from Neuro-2a cells treated with 5 mm metformin for the indicated times were subjected to qRT-PCR using primers specific for OGT and 18 S RNA. *, p < 0.05 compared with 0 h control.