Fig. (1).

Schematic of worm RNAi screens for increased life span. Individual ORFs can be specifically knocked down in C. elegans by feeding worms E. coli expressing double-stranded RNA corresponding to the ORF (RNAi). A library of ~15,000 different RNAi clones has been screened for long life span by identifying RNAi clones that have a high percentage of live animals on them after a length of time sufficient for most control (empty vector = EV) animals to have died. RNAi #2 in this example corresponds to a gene that limits life span.