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. Author manuscript; available in PMC: 2008 Dec 18.
Published in final edited form as: Biochemistry. 2007 Nov 20;46(50):14349–14358. doi: 10.1021/bi701202w

Figure 4.

Figure 4

Separate Western immunoblot assays demonstrate the ability of Fractions 1 and 2 from rHPLC column to inhibit production of TSST-1 by S. aureus MN8. The isoelectric point 7.5 fractions from preparative thin-layer isoelectric focusing were pooled and then subjected to rHPLC, where 5 protein peaks were collected. Only Fractions 1 and 2 contained activity. Arrows indicate the location of TSST-1. Image J density scans of Fraction 1: 1.0 ug/ml 0; 0.1 ug/ml 96.5±2.4 (standard error); 0 ug/ml 104±3.1. Image J density scans of Fraction 2: 1.0 ug/ml 0; 0.1 ug/ml 109±12.3; 0 ug/ml 150±8.2.