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. Author manuscript; available in PMC: 2009 Apr 30.
Published in final edited form as: FEBS Lett. 2008 Apr 7;582(10):1514–1518. doi: 10.1016/j.febslet.2008.03.046

Fig. 2.

Fig. 2

Indole activates the wild-type TRPY1. In whole-vacuole recording mode (A, B) and in excised cytoplasmic-side-out patch mode (C); currents flowing into the cytoplasm are shown downward by convention. A. Ca2+ activation. Increasing [Ca2+] from 10-7 M to 10-5 M Ca2+ (arrow) activated an ensemble of the ~300-pS unitary conductances (in 150 mM KCl). B. Indole activation. Addition of indole in 1% DMSO to the final concentration of 2 mM indole activated the ensemble current as well. C. Unitary conductances were activated in an excised patch with 10-5 M Ca2+ (left segment). Activities subsided after perfusion of 10-7 M Ca2+. At this [Ca2+], the application of 120 mm Hg pressure failed to activate the channel (second segment). The same patch bathed with 1% DMSO control solution did not activate the channel (third segment), but the addition of 2 mM indole did (final segment).