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Separation of peptides resulting from the in-line digestion of 2 pmol of myoglobin in 15 cm × 100 µm I.D. immobilized pepsin reactor using a 20 cm × 100 µm I.D. monolithic column. Myoglobin concentration in solution 1 µmol/L, injection volume 2 µL; Mobile phase: 2% formic acid in water for 25 min pumped through the complete system followed by switch of the valve and use of a 0–80% linear gradient of acetonitrile gradient in 2% aqueous formic acid in 40 min. Flow rate of 500 nL/min.
