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. Author manuscript; available in PMC: 2009 May 15.
Published in final edited form as: J Immunol. 2008 May 15;180(10):6566–6576. doi: 10.4049/jimmunol.180.10.6566

FIGURE 3.

FIGURE 3

Dominant co-stimulation by CD80 results in the generation of hyporesponsive T cells with regulatory function. Co-stimulation blockade assay was carried out as described for Fig. 1 with or without anti-CD80 and/or anti-CD86 Ab. On day 5, CD4+ T cells from these primary cultures were obtained using magnetic bead based negative selection kit, and used in the following experiments. A) Cells were labeled with CFSE, incubated with OVA pulsed APCs for additional 5 days, stained with PE-labeled anti-CD4 Ab and tested for CFSE dilution by FACS. B) Culture supernatants from assay A were tested for cytokines by Luminex multiplex and ELISA assays. CD4+ T cells were gated for panels A and representative values are shown. Mean±SD of values from three individual experiments carried out in triplicate are shown for panel B. This assay was repeated at least 4 times with similar results. * indicates a p value of <0.05.