BIX02188 reverses flow inhibition of TNF-induced signaling. A. BLMEC were exposed to 1 μM BIX02188 for 30 min, flow for 10 min, 10 ng/ml TNF for 15 min (or both). Cell lysates were prepared and SDS-PAGE performed followed by western blotting for p-JNK or total JNK. B. Results represent mean ± S.E. from four independent experiments. Values were normalized by setting the densitometry value of serum-starved cells treated with TNF alone to 1.0 in each experiment. C. Dose-dependent restoration of flow-inhibited TNF-induced JNK activation by BIX02188. Using the experimental protocol described in (A), cells were treated with the indicated concentrations of BIX02188 prior to exposure to flow and TNF. D. The results were quantified after setting the densitometry value of serum-starved cells treated with TNF alone to 1.0 in each experiment. *p < 0.01, compared with cells treated with TNF alone, n=4.