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DNMT mRNA expression is altered with retinoid treatment. (A) DNA methyltransferase (DNMT) expression in T47D cells exposed to 10 μM ATRA over 12 days of concurrent culture. Product bands are shown for DNMT1, DNMT3a, and DNMT3b and the ubiquitous housekeeping enzyme GAPDH. (B) RT-PCR amplification of DNMT mRNAs in T47D breast cancer cells treated with 10 μM 9cUAB30 over 12 days of concurrent culture. Product bands are shown for DNMT1, DNMT3a, and DNMT3b and GAPDH. d, days.
