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. Author manuscript; available in PMC: 2008 Jun 23.
Published in final edited form as: Int J Oncol. 2008 Mar;32(3):625–631.

Figure 4.

Figure 4

Cell viability and apoptosis in response to ATRA treatment. (A) Summary graph of all-trans retinoic acid (ATRA)-induced apoptosis. Percentage of apoptosis determined by Apoptag® Apoptosis assay for HL60 cells treated with 2 μM ATRA. (B) Effects of 2 μM all-trans retinoic acid on HL60 cell viability. Cells were counted on a hemocytometer after trypan blue exclusion staining. Bars represent standard error of the mean from three separate experiments. (C) Fluorescence-activated cell sorting (FACS) analysis of apoptosis after staining with Annexin V-conjugated Alexafluor-488 and propidium iodide. The lower right quadrants contain apoptotic cells. The values indicate representative percentages of apoptosis taken from three separate experiments. Analysis of apoptosis was determined by Apoptag® Apoptosis assay for HL60 cells treated with 2 μM all-trans retinoic acid (ATRA). Brown cells are apoptotic and green cells negative for apoptosis.