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. Author manuscript; available in PMC: 2009 Jan 23.
Published in final edited form as: Dev Cell. 2008 Apr;14(4):559–569. doi: 10.1016/j.devcel.2008.01.019

Figure 5. pm8 migrates ventrally on a laminin tract.

Figure 5

(A) Optical longitudinal section through the middle of a 7 hour embryo stained for laminin. Laminin is present in the basal lamina surrounding the cyst (bracket). A transverse tract of laminin is evident in the posterior, ventral half of the cyst (arrow indicates the cyst midline). (B) lin-12 glp-1 embryo at 7.5 hours. (C–F) Sequence of successively older wild-type embryos showing the appearance (D) and disappearance (E) of the laminin tract, followed by the deposition of laminin on the posterior surface of pm8 after morphogenesis (F). (G–I) Single embryos immunostained for LAM-3 and GFP (ref-1::GFP-PM) before (G), during (H), and near the completion (I) of pm8 migration. Arrows indicate the midline of the cyst. The fixation required for LAM-3 staining compromises GFP-PM localization in pm8 (compare with Figures 4A–D). (J) lam-3; epi-1 double mutant at about 9 hours; pm8 has failed to migrate to the ventral side. This embryo has a shape similar to younger, 7.5 hour wild-type embryos because of defects in body morphogenesis, but has the well-formed tail spike (arrow) characteristic of wild-type 9 hour embryos. (K) ina-1 mutant larva showing pm8 extension into the valve/anterior intestine; similar defects occur in 74% of the hatched animals (n =46). Bars= 10µm (A,B) and 5 µm (G–I).