Quantitative real-time RT-PCR analysis on AP-2γ mutant skin. A) Relative filaggrin mRNA levels were significantly lower in AP-2γ mutant skin when compared to control skin at E16.5, but were not different at E18.5. Bars represent mean ± SD, significance determined by Student t-test, ap<0.05. B) Filaggrin protein monomer levels were determined in E18.5 AP-2γ mutant (lanes 1,2) and control (lanes 3,4) skin by Western blot. Filaggrin levels are reduced in AP-2γ mutant skin. Bars represent mean ± SD, significance determined by Student t-test, ap<0.05.