Figure 6.

Effect of thiamine and benfotiamine on the total thiamine content in thiamine-depleted Neuro 2a cells. The cells were grown for 8 days in thiamine deficient DMEM medium containing 5% fetal calf serum, before addition of thiamine (1 μM, ○) or benfotiamine (1 μM, ●) and intracellular thiamine derivatives were determined at the times indicated. The data (mean ± SEM, n = 3) were analyzed by two-way ANOVA followed by the Bonferroni post-test for comparison of the benfotiamine and the thiamine groups at different times (*, p < 0.05).