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ELISA of purified recombinant LASV proteins using an α-LASV mAb mix. ELISA was performed with 100 ng of purified E. coli-expressed (1) GP2 (GP2 bac), (2) NP (NP bac), and (3) GP1 (GP1 bac). Proteins were incubated with LASV mAb mix, then detected with an HRP-conjugated goat α-mouse IgG antibody and TMB. For negative controls, proteins were incubated with irrelevant mouse IgG (MsIgG) or with an HRP-conjugated goat α-mouse IgG antibody, then detected as above.
