Table 2.
Cross reactivity of Old and New World arenavirus-specific MHAFs against recombinant LASV GP1, GP2, and NP proteins by Western blot and ELISA
| NP bac | GP1 bac | GP2 bac | |||||
| MHAF | Distribution | WB | ELISA | WB | ELISA | WB | ELISA |
| NMS | - | - | - | - | - | - | - |
| LCMV | Old World | +/- | - | - | - | - | - |
| Ippy | Old World | ++ | +/- | - | - | ++ | +++ |
| Mobala | Old World | ++ | ++ | + | + | ++ | ++ |
| Mopeia | Old World | ++ | ++ | - | +/- | + | ++ |
| Latino | New World | - | - | - | - | - | - |
| Tamiami | New World | +/- | - | - | +/- | +/- | +/- |
| Pirital | New World | + | +/- | - | - | - | - |
| Pichinde | New World | + | - | ++ | - | + | +/- |
| Oliveros | New World | +/- | - | - | - | +/- | - |
Western blot (WB) and ELISA were performed with 100 ng of purified E. coli-expressed NP (NP bac), GP1 (GP1 bac), and GP2 (GP2 bac). WB abbreviations: (-) negative, no visible band detected; (+/-) faint band detected; (+) bright band detected; (++) very bright band detected. ELISA abbreviations (all signals are respective to corrected background): (-) negative; (+/-) < 2X; (+) > 2X < 3X; (++) > 3X < 4X; (+++) > 4X < 5X.