Table 3.

Oligonucleotide primers used for amplification of LASV genes expressed in E. coli

LASV Gene Amplified	LASV Primer	Oligonucleotide Primer Sequence
GP1	5' GP1 bac	TTTCAGAATTCGGATCCACCAGTCTTTATAAAGGGGTTTAT
GP1	3' GP1 bac	GGTACCAAGCTTTCAGTCATAGCAATCTTCTACTAATATAAATATCTCT
GP2	5' GP2 bac	TTTCAGAATTCGGATCCGGCACATTCACATGGACACTG
GP2	3' GP2 bac	GGTACCAAGCTTTCAGCTATGTCTTCCCCTGCCTCTCCAT
NP	5' NP bac	TTTCAGAATTCAGTGCCTCAAAGGAAATAAAATCCTTTTTGTGGACACAATCTTTGAGGAGGGAATTATCTGGTTAC
NP	3' NP bac	GGTACCAAGCTTTCAGTTACAGAACGACTCTAGGTGTCGATGT

Note. REN sites are underlined, and stop codons (TCA, CTA, TTA) are in bold print.