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(A) Denaturing high performance liquid chromatography patterns of polymerase chain reaction products corresponding to exon 5 and 7 of ENaCβ : DHPLC profile corresponding to normal exon sequence (control), and altered DHPLC because of a substitution. (B) Fluorescence sequence analysis of exon 5 and exon 7 of the ENaCβ gene, using the forward primer. Arrows indicate position of the p.Asn288Ser and p.Pro369Thr mutations in exon 5 and 7, respectively.
