Figure 10.

Comparison between DNA/DNA and RNA/DNA mismatch binding affinities. (a) Ranking order of DNA/DNA mismatch binding affinities (extracted from Fig. 4). (b) As anticipated the ranking order for DNA/DNA MMs obtained from the smaller subset of probe sequences (Fig. 9A) is very similar. The ranking order for the analogue RNA/DNA MM duplex stabilities (c) (extracted from Fig. 9B) reveals significant differences in comparison to (b). In part (d) MM-types are ordered according to the hybridization signal differences between RNA/DNA and DNA/DNA MMs (as extracted from Additional file 7). Purine-purine MMs (purine bases highlighted in blue) display the largest decrease of binding afinities with respect to other MM-types.