Figure 3.

Phylogram of the chicken PNPLA family. The phylogram was calculated with ClustalW2 using the chicken patatin domain amino acid sequences. The result shows that PNPLAs 1–4 form a subfamily. PNPLA6 and PNPLA7 also form a subfamily, as do PNPLA8 and PNPLA9. The patatin domains of PNPLA2 and PNPLA3 are 68% identical, of PNPLA1 and PNPLA2 51%, of PNPLA1 and PNPLA3 55%, of PNPLA1 and 4 35%, of PNPLA2 and PNPLA4 37%, of PNPLA3 and PNPLA4 40%, of PNPLA8 and PNPLA9 31%, and of PNPLA6 and PNPLA7 76%. Plant patatin was used as an out-group.