Table 2.
Primers used for the cloning of chicken cDNAs.
| Gene | Forward/Reverse | Sequence (5'-3') | Melting temperature (°C) |
| PNPLA1 | Forward | ATGGCTGCAGAGGAGCTGCG | 64 |
| PNPLA1 | Reverse | TTCCTCCAGGCTGGAGACGG | 64 |
| PNPLA2 | Forward | ATGTTCCCTTTGGACTCCGC | 59 |
| PNPLA2 | Reverse | TCAGAAGAGTGGCAGGCACTC | 62 |
| PNPLA3 | Forward | TGGCCAAAGAAGCTAGAAAGCG | 60 |
| PNPLA3 | Reverse | AAGCCTAGGAGGTAGACTCTC | 60 |
| PNPLA4 | Forward | ATGAAACGTGTCAATCTATCATTTG | 56 |
| PNPLA4 | Reverse | CACAGCTATAGGCATTCTGCC | 60 |
| PNPLA6 | Forward | GTGTTGGGACACTTTGAGAAGC | 60 |
| PNPLA6 | Reverse | ATAGATCTCATCGAACTTGC | 53 |
| PNPLA7 | Forward | ATGGAAGAAGAAGGCAACGGCACC | 64 |
| PNPLA7 | Reverse | CTAGGCTGGCTCTCCTGTGCTGC | 68 |
| PNPLA8 | Forward | ATGACAGTTCATTTGTCTCTAG | 55 |
| PNPLA8 | Reverse | GCGTTACGTATTCACAATTTGG | 57 |
| PNPLA9 | Forward | ATGCAGTTCCTCGGGCGGCTTTTG | 66 |
| PNPLA9 | Reverse | TCATCGGCAGAGATACTGCACCAG | 64 |
| CGI-58 | Forward | ATGGCCGAGGAGGAGGCCTCCAG | 70 |
| CGI-58 | Reverse | TCAGTCCACAGAATCACAGATG | 58 |