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. 2008 Jun 22;2008:163902. doi: 10.1155/2008/163902

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Copyright © 2008 Dayanand D. Deo et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Leptin stimulates MAPK phosphorylation in PC-3 and DU-145 cells. Growth factor-starved PC-3 (a) or DU-145 cells (c) were exposed to increasing concentration of leptin for various time points. Cell lysates obtained after each time point were separated on a 7.5% SDS-PAGE and Western-blotted. Phosphorylated MAPK levels were obtained after incubation with p44/42 MAPK antibody and chemiluminescent detection. (b) and (d), levels of p44/42 MAPK obtained after densitometric analysis and represented as percentage of control against time (control being set at 100%). The results of one of the three independent experiments are shown.