Table 1.

Optimisation of BiFC constructs

BiFC pair		Mutation				BiFC fluorescence
N-terminal fragment	C-terminal fragment	L46F	L64F	T153M	Y203H	Xenopus cells	HEK293T cells
VN144	VC145					+	ND
VNm1	VC145	√				+	ND
VNm2	VC145		√			+	ND
VNm3	VC145	√	√			+	ND
VN144	VCm4			√		+	ND
VNm1	VCm4	√		√		+	ND
VNm2	VCm4		√	√		+	ND
VNm3	VCm4	√	√	√		+	ND
VN144	VCm5				√	+	ND
VNm1	VCm5	√			√	+	ND
VNm2	VCm5		√		√	+	ND
VNm3	VCm5	√	√		√	−	ND
VN144	VCm6			√	√	+	ND
VNm1	VCm6	√		√	√	+	ND
VNm2	VCm6		√	√	√	+	ND
VNm3	VCm6	√	√	√	√	−	ND
VN154	VC155					++	ND
VNm7	VC155	√				−	++
VNm8	VC155		√			−	++
VNm9	VC155			√		−	++
VNm10	VC155	√	√			−	±
VNm11	VC155	√		√		−	++
VNm12	VC155		√	√		−	++
VNm13	VC155	√	√	√		−	±
VN154	VCm14				√	−	ND
VNm7	VCm14	√			√	++	ND
VNm8	VCm14		√		√	+	ND
VNm9	VCm14			√	√	++	ND
VN m10	VCm14	√	√		√	+	ND
VNm11	VCm14	√		√	√	++	ND
VNm12	VCm14		√	√	√	+	ND
VNm13	VCm14	√	√	√	√	±	ND