Figure 1.
A, Growth-inhibitory effects of PD0325901 on human thyroid cancer cell lines. The indicated thyroid cancer cell lines were treated for 6 d with PD0325901 (0.2–1000 nm), and the IC50 determined by nonlinear regression using GraphPad Prism version 4 (GraphPad Software Inc., San Diego, CA). B, Growth-inhibitory effects of AZD6244. The indicated cell lines were assessed for response to the MEK inhibitor AZD6244 as described in A, with concentrations ranging from 1 to 5000 nm. C, Basal level of pMEK and pERK in human thyroid cancer cell lines. Western blot of lysates of the indicated thyroid cancer cell lines extracted 72 h after incubation in serum-free media with 0.1% BSA. Membranes were incubated with the indicated antibodies.