Figure 5.

Effect of treatment with AZD6244 on growth of thyroid cancer cell line xenografts in nude mice. A, NPA cells were implanted into the flanks of athymic ν/ν mice, and allowed to reach a volume of about 500 mm³. Mice (n = 9 per group) were then treated with AZD6244 (100 mg/kg, twice a day) by gavage or vehicle. Data represent the mean ± sd of tumor volumes as measured with calipers in the two groups. *, P < 0.02 AZD6244 vs. vehicle-treated mice. At the time the animals were killed, tumor weights were 0.48 ± 0.25 vs. 0.09 ± 0.05, in AZD6244, respectively (P= 0.04). B, Cal62 xenografts were allowed to grow to about 500 mm³ before treatment of mice (n = 4 per group) with vehicle or AZD6244 (100 mg/kg, twice a day) as described in Fig. 6A. Data represent the mean ± sd of tumor volumes as measured with calipers in the two groups. §, P = 0.04, *, P < 0.02 AZD6244 vs. vehicle-treated mice. At the time the animals were killed, tumor weight was 0.4 ± 0.09 vs. 0.09 ± 0.02 g, control vs. treatment, respectively (P = 0.02).