Table 3.

Evolution of the Ly49I repertoire in NK cells over time

Days after pulsing with BrdU*	% BrdU+ NK cells in WT mice†	% BrdU+ NK cells in BCAP−/− mice†	% Ly49I+ NK cells within BrdU+ cells of WT mice†	% Ly49I+ NK cells within BrdU+ cells of BCAP−/− mice†
Day 0	38.4 ± 12.6	38.7 ± 8.0	38.3 ± 2.6	31.8 ± 4.8
Day 14	32.6 ± 6.7	36.8 ± 2.0	39.5 ± 5.0	38.7 ± 2.8
Day 65	6.2 ± 1.9	8.9 ± 1.0	44.1 ± 9.3	54.7 ± 2.5

Each data point represents the mean plus or minus SD from 3 mice.

^*Wild-type and BCAP^−/− mice were pulsed with 5′-BrdU in drinking water for 20 days before day 0. Splenocytes were harvested on the indicated days after pulsing for analysis by flow cytometry.

^†NK cells were gated as NK1.1⁺CD3⁻. The percentage of self-recognizing NK cells was identified by costaining with anti-Ly49I.