Figure 4.

Overexpression of decorin in MSCs via lentivirus-introduced decorin. MSCs were left untreated or were infected with lentivirus-introduced decorin (DCN) or empty vector (EV). (A) Immunohistochemical staining for decorin (original magnification ×200). (B) As determined by qRT-PCR, decorin was overexpressed and overproduced by MSCs infected with lentivirus-introduced decorin. (C) Western blotting revealed overexpression and secretion of decorin core protein by MSCs infected with lentivirus-introduced decorin. (D) Overexpression of decorin had no effect on the ability of MSCs to differentiate into osteoblasts as determined by Alizarin red staining.