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. 1998 Nov 24;95(24):14272–14277. doi: 10.1073/pnas.95.24.14272

Figure 3.

Figure 3

Interaction of pep8 with mutant alleles of Cdk2. (a) LexA fusions to the indicated Cdk2 mutants were tested for interaction with activation-domain fusions of Cdi1, Cks1, p27, p21, pepC, and pep8. Cdk2 mutant alleles are Cdk2-30: V30A, A31F, L32A; Cdk2-33: K33A; Cdk2-38: D38A, E40A; Cdk2-145: D145N; Cdk2-150: R150A, A151F, F152A; Cdk2-159: Y159A, T160D; Cdk2-204: P204A, D206A, E208A, D210A; Cdk2-217: R217A; and Cdk2-250: P250L. (b) Mutations that affect pep8 binding to Cdk2. We made this representation by using rasmol (23) to display the structure of the Cdk2/Cyclin A complex (21) and to delete the cyclinA structure and display the indicated residues. In this figure, Cdk2’s C-terminal lobe is on the bottom, with the active site facing the viewer. Residues that decrease binding to pep8 when they are mutated are shown in purple; those residues that do not affect binding are shown in light gray. The circle denotes the vicinity of the active site; only one mutation that does not affect pep8 binding, Cdk2-K33A, lies within this circle.