Figure 1.

Functional properties of RAD10 prey and LexA-MSH2 bait. (A) Complementation of the UV sensitivity of a rad10 strain by expression of the RAD10 prey protein. The wild-type and rad10 strains were transformed with indicated plasmids and expression of 12CA5-RAD10 protein in the strains was induced by transferring cells into synthetic drop-out (SD) Trp⁻ medium containing galactose followed by shaking for 4 hr. Then 10-fold serial dilutions were prepared and 10 μl of different dilutions were spotted onto SD Trp⁻ plates containing galactose, and UV sensitivity was determined. (B) Complementation of the mutator phenotype of a msh2 strain by expression of the LexA-MSH2 protein. Patches of the indicated strains on a yeast extract/peptone/dextrose plate were replica-plated to a canavanine plate to allow visualization of Can^r papilliae after incubation of the plates at 30° for 2 days.