Figure 2.

Specific interaction of MSH2 with RAD10. The indicated baits and preys were cotransformed into EGY48 along with the lacZ reporter, pSH18–34. The preys are expressed only in the presence of galactose. (A and B) Three different transformants were patched on selective plates and then replica-plated onto 5-bromo-4-chloro-indolyl β-d-galactopyranoside (X-Gal) plates with either glucose or galactose. (C) Patches of one transformant containing each indicated pair of baits and preys replica plated onto X-Gal plates containing galactose. Interaction of a bait and a prey induces β-galactosidase activity producing blue coloration.