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Coimmunoprecipitation of MSH2 and RAD10. Whole-cell extracts (200 μg) were prepared from EGY48 strains expressing LexA-tagged baits (MSH2, or DmHAIRY as a negative control) and 12CA5-tagged preys (RAD10, or ScNPL3 as a negative control). The extracts (E) then were analyzed by immunoprecipitation (IP) and Western blotting. PC, proteins eluted from protein G-Sepharose used to preclear; Ab, antibody; *, proteins nonspecifically precipitated by protein-G Sepharose.
