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Coimmunoprecipitation of MSH2 and different NER proteins. Whole-cell extracts (200 μg) were prepared from EGY48 expressing LexA-tagged bait (MSH2) and 12CA5-tagged preys (RAD10, RAD14, RAD2, RAD25, or ScEXO1 as positive control). The extracts (E) then were analyzed by immunoprecipitation (IP) and Western blotting. PC, proteins eluted from protein G-Sepharose used to preclear; Ab, antibody; *, proteins nonspecifically precipitated by protein-G Sepharose.
