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Interaction of MSH2 and RAD10 in the absence of MSH3, MSH6, MLH1, and PMS1. Whole-cell extracts (200 μg) were prepared from RKY2567 and RKY2752 expressing LexA-tagged bait (MSH2) and 12CA5-tagged prey (RAD10). The extracts (E) then were analyzed by immunoprecipitation (IP) and Western blotting. PC, proteins eluted from protein G-Sepharose used to preclear; Ab, antibody; *, proteins nonspecifically precipitated by protein-G Sepharose.
