Figure 3.

PCR amplification of aggst1α. PCRs were performed on cDNAs from A. gambiae ZAN/U fourth-instar larvae (L), pupae (P), and 1-day-old adults (A) and A. gambiae genomic DNA (B) using the primer sets indicated. The PCR products were separated by gel electrophoresis and transferred to nylon membranes. The DNA was hybridized with a ³²P-labeled 5′ GST probe as described in Methods. The final posthybridization wash was at 65°C in 0.1× SSC and 0.1% (wt/vol) SDS.