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. 1998 Nov 24;95(24):14290–14295. doi: 10.1073/pnas.95.24.14290

Figure 6.

Figure 6

Northern analysis of TTI. (a–c) Northern analysis of RNA purified from adult salivary gland (lane 1), carcasses (salivary gland and midgut removed, lane 2), pupae (lane 3), and larvae (lane 4). a shows the 2% agarose gel stained with ethidium bromide, indicating the presence of ample RNA in all lanes; b shows the hybridization with TTI–cDNA probe; c shows hybridization to mtRNA, again indicating the presence of RNA in the TTI-negative lanes. (d) Tissue-specific and bloodmeal-induced expression of TTI: RNA from teneral midguts (lane 1), from midguts 6 hr after bloodmeal (lane 2), from teneral salivary glands (lane 3), from salivary glands 12 hr after bloodmeal (lane 4), from total gut tissue 12 hr after bloodmeal (lane 5), from midguts 12 hr after bloodmeal (lane 6), and from anterior gut tissue 12 hr after bloodmeal (lane 7). The hybridization probe was TTI–cDNA. (e) The filter in d was hybridized to the mtRNA probe to quantitate the total amount of RNA in each preparation.