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. 1998 Nov 24;95(24):14296–14301. doi: 10.1073/pnas.95.24.14296

Figure 3.

Figure 3

(A) AseI restriction patterns of genomic DNA from S. ambofaciens DSM40697 (WT) and NSA855. Running conditions were: 1% agarose gel, 200 V for 24 h with a ramped pulse time of from 20 to 130 sec for the first three lanes; 0.9% agarose gel, 200 V for 40 h with a ramped pulse time of from 80 to 100 sec for the specific separation of the NA2 fragment (860 kb) and AseI-E (850 kb); and, finally, 1% agarose gel, 200 V for 24 h with a ramped pulse time of from 40 to 160 sec for the last lane. Symbols +, −, and AD91 are as described in Fig. 2. The large fragments in the pronase-untreated lane are slightly degraded. (B) DraI restriction patterns of genomic DNA of S. ambofaciens DSM40697 (WT) and NSA855. Running conditions were: 1% agarose gel, 200 V for 23 h with a ramped pulse time of from 40 to 90 sec. 11D6 indicates Southern blots of the NSA855 pattern probed with labeled 11D6 cosmid DNA. The absence of a hybridization signal on the DraI-I fragment (2,100 kb) is explained by the fact that most of the very large fragments were not transferred to the membrane.