Figure 2.

Effects of monocyte activation on the expression of cell surface death receptors and death receptor-associated genes. Elutriated monocytes were treated with E. coli LPS or a soluble lysate of M. leprae for 6 hr. Total cellular RNA was extracted, and RPAs were performed as described in Materials and Methods. The expression levels of ribosomal L32 and cellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) serve as internal controls as well as RPA performed with yeast tRNA and with RNA derived from monocytes cultured in medium alone (φ, control); similar results were obtained from cells derived from two other donors.