Table 1.
Treatment | % Inhibition of fluorescence* |
---|---|
LPS 25 ng/ml | 86.0 ± 0.4 |
LPS 250 ng/ml | 86.6 ± 0.5 |
*Cell lysates from LPS-treated cells were incubated with IETD-AFC. Free AFC accumulation was monitored by using a fluorometer with 400-nm excitation filter and 505-nm emmision filter. Percent inhibition of fluorescence was calculated in comparison to untreated control samples and represents the mean ± SD of samples done in triplicate.