Figure 1.

Endogenous A72 decreases expansion of 3.L2 T cells in vivo after immunization. (A) 3.L2 splenocytes were transferred by tail vein into A72tg⁺ mice and A72tg⁻ littermates as described in Materials and Methods. Mice were immunized s.c. with 2 nmol Hb(64–76) peptide emulsified in CFA the following day. Five days later, draining lymph nodes were analyzed by two-color FACS for the 3.L2 clonotypic TCR with CAB and for CD4. Histograms plot CD4 gated cells and measure CAB⁺ cells as a percentage of total CD4⁺ cells. (B) Mice were handled identically to those in A with the exception that a higher 20 nmol dose of Hb(64–76) peptide was given. (C) Experiment was performed identically to that in A with the exception that E72tg⁺ and E72tg⁻ mice were used as recipients. Data in each panel are representative of four to eight pairs of mice.