Figure 2.

3.L2 T cells establish in similar numbers and maintain equal proliferative ability in A72tg⁺ and A72tg⁻ recipients. (A) Equal numbers of unpurified 3.L2tg splenocytes were transferred to A72tg⁺ and A72tg⁻ recipients as described. The percentage of CD4⁺CAB⁺ T cells was quantitated by FACS in spleen and popliteal plus superficial inguinal lymph nodes 4 days later and is shown as the mean of values from three mice in each group. (B) Three spleens in each group from A were pooled and depleted of antigen presenting cells over nylon wool columns. Purified T cells (4 × 10⁵ per well) were added to irradiated B6.AKR splenocytes (5 × 10⁵/well) in the presence of the indicated concentrations of Hb(64–76) peptide. Proliferation was measured as ³H thymidine incorporation after pulsing at 48 hr and harvesting at 72 hr. Data are representative of two identical experiments.