Table 1.

Mutations are targeted to the CDRs in the Sec clones and display evidence of positive selection in CDR1

Region	% of analyzed sequence	% mutations*	R/S†	P̄n (SD)/P̄s (SD)*
Sec
FR1	25%	8.6%	11/7	0.028 (0.03)/0.047 (0.05)
FR3	38%	24%	40/12	0.020 (0.02)/0.019 (0.02)
CDR1	13%	34%	61/10	0.097 (0.05)/0.073 (0.12)‡
CDR2	10%	24%	43/8	0.109 (0.082)/0.126 (0.13)
CDRs total	23%	58.9%  ±  7.9§
Tm
FR1	25%	15%	14/3	0.02 (0.012)/0.01 (0.033)
FR3	38%	38%	26/9	0.02 (0.012)/0.03 (0.029)
CDR1	13%	25%	18/7	0.06 (0.052)/0.09 (0.105)
CDR2	10%	9.9%	6/2	0.05 (0.024)/0.09 (0.123)
CDRs total	23%	34.7%  ±  8.6

^*Mutated clones with fewer than 15 mutations (Sec, n = 66; Tm, n = 60). 

^†Excludes tandem mutations where the type of change depends on which mutation occurred first. These were not excluded from the Pn, Ps analysis. R, replacement; s, silent. 

^‡P < 0.05 (Wilcoxon Signed-Ranks Test). 

^§P < 0.05 (G test; Sec clones CDR mutation frequency was higher than Tm clones).