Figure 3.

Effects of the NP34 transgene on the expression of the F5 TCR on thymocytes. Cells from positively selecting F5⁺Rag-1^−/−Tap-1⁺ (A), nonselecting F5⁺Rag-1^−/−Tap-1^−/− (B), and negatively selecting 5⁺Rag-1^−/−Tap-1⁺I-E⁺ (E) thymuses were compared with cells from F5⁺Rag-1^−/−Tap-1⁺NP34⁺ (C) and F5⁺Rag-1^−/−Tap-1^−/−NP34⁺ (D) thymuses. Cells were stained with anti-Vβ11 phycoerythrin antibodies and analyzed by FACS. Histograms represent the fluorescence intensity of Vβ11.