Figure 4.

(A) The NP34 transgene reduces the percentage of CD69⁺ thymocytes. Cells were isolated from F5⁺Rag-1^−/−Tap-1⁺NP34⁺, F5⁺Rag-1^−/−Tap-1^−/−NP34⁺, positively selecting F5⁺Rag-1^−/−Tap-1⁺, nonselecting F5⁺Rag-1^−/−Tap-1^−/−, and negatively selecting F5⁺Rag-1^−/−Tap-1⁺I-E⁺ thymuses. Cells were stained with anti-CD69 phycoerythrin antibodies and analyzed by FACS. Bars represent the mean percentage of CD69⁺ thymocytes from three to eight mice of each genotype. SDs are indicated. (B) The NP34 transgene has no influence on the absolute numbers of thymocytes. Cells from F5⁺Rag-1^−/−Tap-1⁺NP34⁺, F5⁺Rag-1^−/−Tap-1^−/−NP34⁺, positively selecting F5⁺Rag-1^−/−Tap-1⁺, nonselecting F5⁺Rag-1^−/−Tap-1^−/−, and negatively selecting F5⁺Rag-1^−/−Tap-1⁺I-E⁺ thymuses were isolated and counted. Bars represent the mean thymocyte numbers from three to eight mice of each genotype. SDs are indicated.