. 1998 Nov 24;95(24):14349–14354. doi: 10.1073/pnas.95.24.14349

Table 1.

Effects of NP34 or I-E on the number of F5⁺CD8⁺ SP T cells

Genotype	Spleen	Lymph nodes
F5⁺Rag-1^−/−Tap-1⁺	7.5 ± 2.8 × 10⁶	3.6 ± 1.8 × 10⁶
F5⁺Rag-1^−/−Tap-1⁺NP34⁺	1.4 ± 0.37 × 10⁶	0.59 ± 0.47 × 10⁶
F5⁺Rag-1^−/−Tap-1⁺I-E⁺	0.9 ± 0.45 × 10⁶	0.14 ± 0.095 × 10⁶

The NP34 transgene reduces the absolute numbers of CD8⁺ splenocytes and lymph node cells. Cells from F5⁺Rag-1^−/−Tap-1⁺NP34⁺, positively selecting F5⁺Rag-1^−/−Tap-1⁺, and negatively selecting F5⁺Rag-1^−/−Tap-1⁺I-E⁺ lymphoid organs were isolated and counted. Cells were stained with anti-CD8 antibodies and analyzed by FACS. The absolute numbers of CD8⁺ T cells were calculated by multiplying the total cell number per organ with the percentage of CD8⁺ cells. Mean numbers of CD8⁺ T cells from six to eight mice of each genotype are shown. SDs are indicated.