Table 1.
Experiment | Animal no.* | Species | Sex† | Antibody positive | Tissues PCR positive | Time of death, D or sacrifice, S |
---|---|---|---|---|---|---|
1 | S. hispidus | F | p17, p24 | − (negative) | S, wk 52 | |
2 | S. fulviventer | M | p17 | — | S, wk 24 | |
3 | S. hispidus | M | p24 | — | S, wk 24 | |
4 | S. hispidus | M | — | PBMC† | S, wk 24 | |
5 | S. fulviventer | M | p51, p55 | PBMC | S, wk 52 | |
No. 1 | 6 | S. fulviventer | F | p17 | SPL, LN, BM | S, wk 24 |
7 | S. hispidus | M | — | — | S, wk 52 | |
8 | S. fulviventer | F | p24 | — | S, wk 52 | |
9 | S. fulviventer | F | p17, p24, p51, p55 | PBMC | S, wk 52 | |
10 | S. fulviventer | M | p51, p55 | PBMC, SPL | S, wk 52 | |
11 | S. fulviventer | F | p17, p51, p55 | PBMC, SPL, THY | S, wk 52 | |
12 | S. hispidus | F | — | — | D, day 0 | |
23 | S. hispidus | F | — | PBMC | D, wk 42 | |
24 | S. hispidus | F | — | PBMC | S, wk 60 | |
26 | S. hispidus | M | — | — | D, day 9 | |
27 | S. hispidus | M | — | — | D, day 0 | |
28 | S. hispidus | M | — | — | D, day 0 | |
No. 2 | 29 | S. hispidus | M | — | — | D, day 0 |
30 | S. fulviventer | F | p17 | PBMC | S, wk 60 | |
31 | S. fulviventer | F | — | PBMC, BM, THY | S, wk 60 | |
32 | S. fulviventer | M | — | BM | S, wk 60 | |
33 | S. fulviventer | M | — | BM, SPL | S, wk 60 | |
34 | S. fulviventer | M | — | — | S, wk 60 | |
35 | S. fulviventer | F | — | — | D, day 0 |
Animal nos. 1–12 were from experiment no. 1 and animals nos. 23–24, 26–35 were from a confirmatory experiment (no. 2) as described in Materials and Methods. F, female, M, male.
In most cases at killing or death, the following tissues were taken for histology and PCR analysis: abbreviations; SPL, spleen; BM, bone marrow (femur); THY, thymus; LN, lymph node. Brain, lung, and kidney also were sampled but none were positive by PCR-SB and so are not included in the table.