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Southern blotting using probes flanking the transgene. DNA from +/+, +/−, or −/− animals was digested with EcoRI and hybridized with each end of a 3.9-kb EcoRI fragment containing the transgene insertion site (see text). (A) One end detects an altered fragment (2.8 kb) for the morc chromosome. (B) The opposite end detects the 3.9-kb wild-type fragment with half intensity in +/− animals and no fragment in −/− mice, demonstrating a deletion to one side of the transgene.
