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. 2008 Feb 25;153(7):1538–1549. doi: 10.1038/bjp.2008.33

Figure 4.

Figure 4

PGE2-G was hydrolysed into PGE2 and other unknown metabolites after it was injected into the rat hind paw. (a) Levels of PGE2-G following its injection into the rat hind paw. Hind paws were removed at 0, 15, 30, 60 or 120 min following PGE2-G injection (50 μg 50 μl−1, i.pl.), extracted, partially purified and subjected to LC/MS/MS to measure the level of PGE2-G. At 0 and 15 min, only 6.8 and 1%, respectively, of the injected amount of PGE2-G were recovered (***P<0.0001, **P<0.001). The amount of PGE2-G fell to the baseline level 30 min after injection. (b) Rat hind paws were removed at 0, 15, 30, 60 or 120 min after PGE2-G injection (50 μg 50 μl−1, i.pl) and the levels of PGE2 were assessed by LC/MS/MS. Immediately after injection (0 min), approximately 10% of the injected amount of PGE2-G was hydrolysed into PGE2 and the level of PGE2 then decreased with time (***P<0.0001, *P<0.05). i.pl., intraplantar; LC, liquid chromatography; MS, mass spectrometry; PGE2, prostaglandin E2; PGE2-G, prostaglandin E2 glycerol ester.