Figure 7.

Rhein had no direct effect on GFAT enzymatic activity. MCLacZ cells were normally cultured as above. When the cells were 80% confluent, the cells were scraped into GFAT buffer and the cellular supernatant, which contains GFAT, was obtained. Rhein (5–50 μg ml⁻¹) was added directly to 100 μl of GFAT containing cellular supernatant and the GFAT activity was then measured as described above. Each value represents the mean±s.e.mean of six samples. GFAT, glutamine:fructose 6-phosphate aminotransferase.