Figure 3.

The binding capacity and pharmacological profile of [³H]-silodosin and [³H]-prazosin binding sites in rat cerebral cortex (a) and tail artery (b). High- and low-affinity sites for prazosin at [³H]-silodosin binding sites were represented as α_1A (A) and α_1L (L), respectively. Likewise, the number of α_1A (A) and α_1B (B) ARs was estimated from the proportions of high- and low-affinity sites for silodosin at [³H]-prazosin binding sites, respectively (see the text for further details). B_max values represent the mean±s.e.mean of 4–5 experiments. Segments: intact tissue segment binding. Membranes: crude membrane binding. ^*Significantly different from [³H]-silodosin binding sites (P<0.05). B_max, maximal binding capacity.