Skip to main content
. 2008 Feb 18;153(7):1409–1419. doi: 10.1038/bjp.2008.21

Figure 3.

Figure 3

Effects of rosiglitazone (Ros) and 15d-PGJ2 (15d) on Ang II-induced TGF-β1/Smad2/3 and JNK signalling pathways in cardiac fibroblasts. Cells were pretreated with 5 μM rosiglitazone or 15d-PGJ2 for 1 h, and then stimulated with 0.1 μM Ang II for 24 h. Western blot analysis was subsequently used for detecting the expression of TGF-β1, Smad2/3 and JNK protein; cells were pretreated with 5 μM rosiglitazone or 15d-PGJ2 for 30 min, followed by 0.1 μM Ang II incubation for 20 min. Phosphorylated proteins were then detected. Rosiglitazone and 15d-PGJ2 inhibited Ang II-induced TGF-β1 expression (a), Smad2/3 (b) and JNK (c) expression and phosphorylation. All values are showed as mean±s.e.mean of three experiments. *P<0.05 vs control; †P<0.05 vs Ang II. Ang II, angiotensin II; 15d-PGJ2, 15-deoxy-Δ12,14-prostaglandin J2; JNK, c-Jun NH(2)-terminal kinase; TGF-β1, transforming growth factor-β1.