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Comparison between direct, real-time MSP and nested, gel-based MSP results. Box-plots and strip-charts compare the log2(1000 * m_MGMT/ACTB) (y axis) values determined for 91 samples by direct, real-time MSP assay to the classification obtained with the nested, gel-based assay into non-methylated (green) and methylated (red) samples (x axis). A dashed line represents the cutoff defined with the Gaussian mixture model (Figures 3 and 4).
