Fig. 1.

Two peptides of different size translated from a single Opn mRNA species. (A) Opn detection from pDC lysate by immunoblotting with O-17 Ab. (B) Transcripts from “Full” (Opn-fl) and “OpnΔ48Koz.” Triangles (▵) denote AUG codons, including the second endogenous AUG at position 84 aa. O-17 Ab recognizes amino acids spanning position 17–32 aa. (C) IVT of two Opn constructs shown in B. Peptide products were phosphatase-treated. Negative control of IVT was performed without RNA template (“no RNA”).